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Apoptosis

Apoptosis

Apoptosis, or programmed cell death, refers to a series of biochemical changes in the cell leading to distinct morphological changes that can be assessed using fluorescent probes or contrast-enhancing microscopy modes such as phase contrast. Examples of fluorescent probes include those that target phosphatidylserine which becomes exposed to extracellular reagents in early stage apoptosis (i.e. pSIVA-IANBD), fluorometric substrates of or antibodies to caspases (i.e. Caspase 3/7) that generate fluorescence with caspase activation, or probes that measure chromatin condensation. Cytation™ and Lionheart™ FX both have the ability to measure apoptosis through the use of fluorescent probes or by label-free methods relying on phase contrast microscopy.

 

20x Kinetic Apoptosis and Necrosis Movie
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Application Notes:

 

Visual Abstracts:

BioTek’s Visual Abstracts are brief animated presentations describing the Augmented Microscopy workflow of a specific application.

Measuring Apoptosis in Zebrafish Treated with Ethanol
Measuring Apoptosis in Zebrafish
Treated with Ethanol

Live Cell Imaging of Apoptosis and Necrosis
Live Cell Imaging of Apoptosis and Necrosis

Live Cell Imaging of Apoptosis and Necrosis
3D Kinetic NK Cell Cytotoxicity Assay

 

Related Products:

Learn more about Apoptosis applications - search our Application Notes and technical documentation in Resources.

 

 

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