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Combining Automated Liquid Handling, Cellular Microscopy and Magnetic Bioprinting to Create Walk Away 3D Oncology, Stem Cell Differentiation, and Toxicity Procedures
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May 24, 2017
Presenters: Jan Seldin - Greiner Bio-One, Monroe, NC; Glauco Souza - n3D Biosciences, Inc., Houston, TX; Brad Larson - BioTek Instruments, Inc., Winooski, VT
The use of cells cultured into a three-dimensional (3D) configuration continues to gain acceptance within multiple areas of life science research and drug discovery. Assay procedures commonly performed with two-dimensional (2D) cultured cells are rapidly migrating to 3D because of the reestablishment of cell-cell and cell-extracellular matrix (ECM) interactions, and in vivo-like results these cell models afford. Examples include cell migration studies, stem cell differentiation, and toxicity assessments. Magnetic bioprinting is a versatile method for aggregating cells and fomenting ECM interactions that can be used across multiple assay procedures. Using this technology, cells can be printed into 3D ring, dot, or spheroid structures after biocompatible magnetic particles are attached. In this webinar we will demonstrate how assay procedures using 3D magnetic bioprinting can be performed in a walk-away manner using automated liquid handling and incubation, and monitored through fluorescent and label-free image-based analysis.