product design

Scratch Assay Starter Kit
for Wound Healing and Cell Migration Assays

Part Number: 1750012

用於: Lionheart FX, Cytation 5, Cytation 1, Scratch Assay App

Scratch Assay Starter Kit <br>for Wound Healing and Cell Migration Assays

BioTek’s Scratch Assay Starter Kit includes everything a researcher needs to implement automated, kinetic cell migration and scratch wound assays, in conjunction with a BioTek Lionheart™ Automated Microscope or Cytation™ Cell Imaging Multi-Mode Reader.


Kit-Box-Lid-Open-ContentsEach Scratch Assay Starter Kit includes:

  • BioTek’s AutoScratch™ Wound Making Tool to automatically create repeatable scratch wounds
  • The Scratch Assay App software: Automatically calculates key statistics including wound width, % confluence and maximum healing rate
  • Sample packages of 24-well and 96-well microplates
  • Reagents to ensure the best scratch performance, assay repeatability and easy maintenance

Other available accessories for scratch assay workflows include:
  • PN 02682 - Case of 100 (individually wrapped) Corning® Costar® 24-well Clear TC-Treated Plates - flat bottom, polystyrene, sterile, lid
  • PN 02683 - Case of 100 (20 packs of 5) Corning® 96-well Clear TC-Treated Plates - flat bottom, polystyrene, sterile, lid
  • PN 1750006 - Set of 8 spare scratch pins for AutoScratch Wound Making Tool
  • PN 1753008  - Cleaning reagents for AutoScratch Wound Making Tool

Application Notes:  

Product Videos:

Scratch Assay
BioTek’s Scratch Assay Starter Kit includes everything a researcher needs to implement automated, kinetic cell migration and scratch wound assays.
Automated Wound Creation
This is a tutorial video about optimizing your microplate-based scratch migration assays by effectively washing samples after scratches have been formed. Post-scratch washing is an important step in the wound healing assay protocol, whether you are generating your scratches manually, or using the fully automated BioTek AutoScratch Wound Making Tool to efficiently create uniform scratches in either 24- or 96-well microplates.
Automated Wound Creation
Upon completion of automated wound creation using the AutoScratch tool, automated plate washing can be achieved using the MultiFlo FX to remove cells dislodged from the plate bottom. A series of three plate washes are completed in a sterile environment followed by media and/or inhibitor addition prior to performing cell migration studies. Learn more at www.biotek.com/multiflofx.  


Visual Abstracts:

Automated Wound Creation
Automated Scratch Wound Assay Wash Step
using MultiFlo FX Multi-Mode Dispenser

 

FEATURES

green box Convenient, all-inclusive kit

 All-inclusive kit guarantees setup in minutes with no training required

The Scratch Assay Starter Kit provides everything you need to rapidly implement scratch wound assays with your BioTek imager.

1. AutoScratch Wound Making Tool
2. Sterile 96 and 24-well microplates
3. Scratch pin cleaning solutions
4. Scratch Assay App

green box  Highly consistent scratch wounds

Higher quality data

AutoScratch automatically creates wounds with high consistency from plate to plate.

  • Wound width %CV < 2%
  • Vastly improved over manual scratch assay

green box  Pre-defined protocols

Automatic % live/dead calculation using trypan blue stain
The Scratch Assay App includes customizable, pre-defined protocols that calculate results automatically.
 

green box  24- and 96-well plates

Fast cell count for up to 8 samples per batch
The kit includes 24- and 96-well plates to facilitate scratch assay implementation in both plate types.
 

green box  Automated cleaning and decontamination

Built-in Dilution Calculator facilitates downstream applications

AutoScratch features automated routines for unattended maintenance. Reagents are included.

1. AutoScratch automatic cleaning area
2. Supplied cleaning reagents

 


 


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