product design

Lionheart FX 自動化活細胞影像系統

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Lionheart FX automated microscope is a compact system for a broad range of imaging workflows. It offers up to 60x air; 60x and 100x oil immersion magnification, with fluorescence, brightfield, color brightfield, and phase contrast channels for maximum application reach. An optional environment control cover provides incubation to 40 °C and effective containment for CO2/O2 control. A humidity chamber optimizes conditions for long-term live cell imaging applications, and an available dual reagent injector facilitates rapid kinetic assays.


刮 Scratch分析入門套組 與BioTek的Lionheart FX自動化活細胞影像系統或Cytation 5配合使用,可自動進行細胞遷移和傷口癒合測定。

特別優惠 !購買Lionheart或Cytation,即可免費獲得獲得專利的雷射自動對焦組!


Lionheart FX is designed for easy implementation of a broad range of fixed and live cell assay workflows. BioTek’s concept of Augmented Microscopy™ brings automated image capture, processing, analysis and publication-ready images and data to the benchtop – for many applications, including these important areas:

Live Cell Proliferation and Viability Application Guide
Live Cell Proliferation and Viability


The Scratch Assay Starter Kit automates cell migration and wound healing assays:

AutoScratch automatically makes consistent, repeatable scratch wounds

AutoScratch automatically makes consistent, repeatable scratch wounds.

The Scratch Assay App automatically calculates % confluence,  wound width and maximum healing rate (max V)

The Scratch Assay App automatically calculates % confluence, wound width and maximum healing rate (max V).

The Scratch Assay Starter kit enables automated scratch wound assays with excellent performance, compared to manual methods.

AutoScratch creates scratch wounds with excellent performance, compared to manual methods.


Adding 10 µM ATP activates HeLa endogenously expressed P2Y receptor

Adding 10 µM ATP activates HeLa endogenously expressed P2Y receptor

Rat kidney mitosis

Rat kidney mitosis, prophase at 60x.


Visit our Applications pages to learn more about all of the critical applications enabled with Lionheart FX.

BioTek Field Applications Scientists (FAS) and Product Specialists provide unparalleled scientific support, assisting with experimental planning and assay optimization on BioTek’s instrumentation and software. Learn how they can help move your science forward… faster!



   Automated digital microscopy up to 100x

 Automated digital microscopy to 100x

Lionheart FX with Augmented Microscopy automates your imaging workflow to yield amazing images and powerful data. Automated image capture starts with image-based and laser autofocus, plus auto LED and exposure. Automated image pre-processing optimizes images for downstream analysis, from cell counting to characterization of subcellular details.


   Four imaging modes, multiple imaging processes


With brightfield, color brightfield, phase contrast and fluorescence imaging in four channels – and nearly twenty available filter/LED cubes, Lionheart FX offers fantastic versatility that isn’t limited to image capture. Follow your imaging processes through z-stacking to z-projection, montage collection to stitching and digital phase contrast to analysis, all one hardware and software platform.

Four imaging modes, multiple imaging processes

   Label-free cell counting

 Label-free cell counting

It’s fast and simple to count live cells using the available high contrast brightfield option. Higher contrast allows a direct and label-free method for object identification using Gen5 software to count cells and characterize cell proliferation in kinetic studies.


   Kinetic live cell assay support


Several options are available to support live cell assays with Lionheart FX, including an environmental cover to maintain temperature and gas levels within the system. Long-term live cell kinetics are supported, using the unique humidity chamber that fits on the stage itself, keeping the live cell in a completely nurturing environment. A dual reagent injector provides quick inject/image capability for observing fast reactions.

Automated digital microscopy to 100x

   More than 20 imaging filter/LED cubes

Four imaging modes, multiple imaging processes

BioTek has the most comprehensive selection of imaging filter and LED cubes available for use with our Lionheart and Cytation imagers. This large selection of colors enables image capture for a broad range of applications. Download our convenient Imaging Cube Selection Guide here.


   Quick installation and easy setup


Fifteen minutes is all it takes to install and set up Lionheart FX for image capture and analysis. The filter/LED cubes and objectives are accessed from the front panel, and tool-free connections for injectors, gas control and the environmental cover add to the easy installation. Its small size takes up minimal benchtop space.

Label-free cell counting



Discovering New Ways to Advance Cell Biology Research with Lionheart FX


Creating an in vitro environment that is highly similar to that found in the body, including the presence of an extracellular matrix (ECM), is a critical aspect of reaping the most relevancy from cell-based research. Often, researchers find that optimizing culture environments is a time-consuming and costly process. The team at denovoMATRIX GmbH in Dresden, Germany, seeks to ease this burden by developing and manufacturing novel, pre-coated cultureware for specific cell types. Dr. Dejan Hušman,  CEO of denovoMATRIX,  explains that the specialized coatings used on the cultureware are composed of glycosaminoglycans and biomimetic peptides that can be combined to generate entire libraries of extracellular matrices for use in their user-customized myMATRIX and their flagship screenMATRIX products. Dr., Hušman  notes that development of the latter was dependent on their use of BioTek’s Lionheart™ FX Automated Microscope.




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