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Automated Cytotoxicity Profiling Using the EL406 Washer Dispenser with CELLestial™ Assay Kits for Microplates下載
Related Products: EL406 多功能微量盤清洗及分注儀
January 26, 2010
Authors: Paul Held, Peter Banks, BioTek Instruments, Inc., Winooski, VT, USA; Dee Shen, Wayne Patton, Enzo Life Sciences, Farmingdale, NY, USA
Almost all compounds will elicit deleterious effects on cells at some concentration. Anticancer agents typically have cytoxicity of tumor cells as a primary function, while medicinal agents can have cytoxicity as an unwanted side effect. Drugs elicit cytotoxic effects through several different mechanisms, which can be discerned by a variety of assays. Drug accumulation in the mitochondria can result in a compromised membrane potential with a resultant disruption in electron transport. Lysosome perturbation, as a result of ion trapping of amine containing compounds has been demonstrated to cause the formation of autophagosomes and autophagic cytopathology. Additionally numerous compounds have been shown to initiate the apoptotic process in cells. Here we describe the use of the EL406 Washer Dispenser to automatically aspirate media, wash cells and dispense reagents for three different CELLestial™ cytotoxicity detection assays from Enzo Life Sciences. H-Meso cells have been treated with agents know to perturb lysosomes, such as chloroquine and verapamil and their effects monitored using the Lyso-ID™ Red Detection Kit. Likewise, the effect of staurosporine in regards to the induction of apoptosis has been assessed using the Nuclear-ID™ Green Chromatin Condensation Kit. Mitochondrial membrane toxicity has been monitored using the Mito-ID™ Membrane Potential Cytotoxicity.