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Efficacy of Using a Combination Microplate Washer for Vacuum-Based DNA Sequencing Reaction Cleanup下載
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July 01, 2011
Authors: Wendy Goodrich, Andreas Rieger, BioTek Instruments, Inc., Winooski, Vermont, USA; Mary Louise Shane, DNA Analysis Facility at the Vermont Cancer Center, Burlington, Vermont, USA
The ability to determine the specific pattern of base pairs in DNA molecules is an indispensable part of contemporary molecular biology. Over the past 10-12 years the evolution of market leading dye terminator methods and automated capillary electrophoresis instrumentation has largely standardized the procedure for DNA sequencing, quickly making it more accessible, less resource intensive, and easier to perform at many different throughput levels. A critical component of this genomic workflow is the sequencing cleanup procedure, where contaminating artifacts of the sequencing reaction are removed prior to capillary electrophoresis. There are currently a number of viable DNA sequencing cleanup methods available using either filtration, precipitation, or sequestering as a process of choice. Each method has its own costs and benefits and is a proven way of purifying reaction samples. Where dedicated instrumentation is not required, value can be gained by the use of multi-functional instruments that can perform tasks across many application areas, contributing to optimal resource sharing typical of many molecular biology laboratories and core facilities. In collaboration with a comprehensive DNA Analysis Core Facility using state-of-the-art sequencing chemistries and instrumentation we demonstrate an example of the merit of hybrid instrumentation by using an integrated vacuum filtration module of a combination micro plate washer to perform DNA sequencing reaction cleanup.
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