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Fast Monitoring of Growth of Industrial Yeast Cultures by Synergy H1下載
Related Products: Gen5 微量盤偵測軟體特色, Synergy H1 多功能微量盤檢測儀
May 06, 2011
Authors: Gary Prescott, BioTek Instruments, Inc., Luzern, Switzerland; Anita Van Landschoot, University College Ghent and Ghent University, Ghent, Belgium
Strains of the yeast Saccharomyces serve critical roles in the production of many beverages, but also in the production of ethanol for biofuel. The ability to obtain the desired product reliably and repeatedly in the same amounts requires careful monitoring of not only the input materials, but also the growth of the yeast strain during the propagation or the hydratation/revitalization in the case dried yeast is used for the fermentation process. Likewise this often requires monitoring the growth of these strains under various conditions to optimize the industrial conditions. Here we describe the use of the Synergy™ H1 Hybrid Multi-Mode Microplate Reader to provide temperature control, suspension agitation, and monitor cellular yeast growth using light scatter in 96-well microplates at 600 nm. Measurements were made every 2 minutes and data are collected using Gen5™ Data Analysis Software (BioTek Instruments, Inc).