更多資源 - 科學海報
Optimization of a multi-mode detection model for measuring real-time cellular respiration and mitochrondrial function using fluorophoric biosensors下載
Related Products: Cytation 1自動化影像系統暨多功能光學檢測儀 , Gen5 微量盤偵測軟體特色, Synergy H1 多功能微量盤檢測儀, Synergy Neo2 多功能微量盤檢測儀
February 13, 2015
Authors: Wendy Goodrich, Xavier Amouretti, BioTek Instruments, Inc.; Conn Carey, Luxcel Biosciences
Characterization of cellular metabolism is being aided by the development of new tools designed to provide ease-ofuse, higher throughput, and multiplexed data markers for analysis. One of these tools is a simple mix and measure assay compatible with a variety of cellular matrices that utilizes fluorophoric probes to measure oxygen consumption rates (OCR), extracellular acidification (ECA), and intracellular oxygen levels useful to inform on the activity of the electron transport chain (ETC) and glycolytic flux. These probes can be detected using standard fluorescence, time-resolved fluorescence, or lifetime fluorescence with reduced background and increased signal dynamic range dependent on the detection mode. Optimization of biosensor recognition in all three fluorescent modes was done in microplate format using multiple cell lines and drug compound treatments. In particular, the lifetime time-resolved fluorescent mode is highlighted for generating drug compound dose response against OCR (μs/hr), presenting accurate comparisons of acidification rates converted to hydrogen ion scale (ECA[H+]/t), and detecting intracellular oxygen levels in parallel with fluorescent imaging in live cell 2D monolayers.
Cytation 3 Cell Imaging Multi-Mode Reader