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Sub-Micron Spot Quantitation of Centromeres
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July 28, 2017
The resolving power of an imaging system is the foundation of successful data analysis. This experiment compares a 20x air objective(0.45 NA)to a 100x oil immersion objective(1.42 NA)for the count of sub-micron objects (centromeres) in cell nuclei. Cell nuclei are stained with DAPI and centromeres (CENP-A) areimmunostainedwith Texas Red-labeled antibodies.